GLO1 and breast cancer: By comparing control and GLO1-depleted cells, we identified 47,578 differentially methylated CpGs (DMCs) accounting for 22,702 genes, among which the large majority (41,431 DMCs; 87.1%) was hypermethylated in shGLO1 cells (Fig. 1A, Data S1) indicating a significant expansion of genome methylation upon GLO1 depletion in breast cancer cells.