Hypermethylation affected promoter and/or enhancer regions of specific TSGs known primarily for their role in regulating the metastatic potential of cancer cells such as: ENTPD3, an inhibitor of EMT and metastasis in breast cancer [66], and ALCAM cell adhesion molecule which reduced expression has been associated with poor prognosis in a large TMA-based immunohistochemical analysis of breast cancer [67]. The gene discussed is ALCAM; the disease is breast cancer.