Representative GSEA enrichment plots are shown in Fig. 1E. The second category of the main hypermethylated pathways under study, namely TSG-A, pointed to gene sets related to classic TSGs such as RB, P53 and PTEN thus, inferring to a regulatory mechanism occurring under elevated MG condition that targets TSGs primarily known to be down-regulated/inactivated through mutation in cancer. This evidence concerns the gene RB1 and cancer.