To study the biological function of CD248 in OS cells, we first examined the expression level of CD248 in eight different sarcoma cell lines, and found that SJSA-1, HOS, A-673, SK-N-AS cells had relatively high CD248 expression, while CD248 expression was relatively low in 143B, MG63, Saos2 and U2OS cells (Fig. S1A-S1B). The gene discussed is CD248; the disease is sarcoma.