N-ethyl-N-nitrosourea (ENU) mutagenesis studies have created mice with spontaneous mutations in Plcg2. The initially described model was the Ali5 mouse, which carried a heterozygous gain-of-function mutation in Plcg2 at p.Asp993Gly19, leading to severe spontaneous inflammation and autoimmunity. This evidence concerns the gene PLCG2 and Autoimmunity.