For the BE3 and YE1-BE3-FNLS mouse lines, we constructed a U6-Hpd sgRNA vector containing an sgRNA for C-to-T editing of the Hpd locus (Supplementary Table 3), which results in a stop codon that can rescue the lethal phenotype of hereditary tyrosinemia type 1 in mice17. The gene discussed is HPD; the disease is Tyrosinemia type 1.