To investigate whether SLC7A11 reconstitution can prevent the enhancement of liver damage and tumour formation in ATF4-deficient mice, we used adeno-associated virus 8 (AAV8) to transduce SLC7A11, whose N-terminus was fused to mCherry (AAV8-xCT), or mCherry (AAV8-mCherry), into livers of 8-wo MUP-uPA/Atf4F/F and MUP-uPA/Atf4Δhep mice (Fig. S7B). Here, ATF4 is linked to neoplasm.