Similar to MUP-uPA mice,7 HFD-fed 6-mo MUP-uPA/Atf4F/F mice displayed typical NASH signs, including immune infiltration and fibrosis, which were more pronounced in MUP-uPA/Atf4Δhep mice, that also presented with liver tumour nodules and high circulating ALT (Fig. 5A–E). This evidence concerns the gene PLAU and metabolic dysfunction-associated steatohepatitis.