The genes related to cancer including ABCA5, CDKN1B, CEBPB, FGFR2, FLCN, FZD1, LMO4, LPAR6, ROCK1 and VEGFA were highly expressed in CC organoids and were also upregulated in HB-tumor organoids when compared to HB non-tumor organoids. This evidence concerns the gene FLCN and cholangiocarcinoma.