Further KEGG Pathway enrichment bubble diagram (Fig. 7b) showed that the differential genes were enriched in a variety of cancer signaling pathways, including Viral carcinogenesis, Neutrophil extracellular traps, Systemic lupus erythematosus, etc. Combined with KEGG Pathway classification analysis and enrichment analysis, B3GNT3 may be involved in a variety of carcinogenesis pathways in ESCC cells by regulating the target genes of mRNAs. This evidence concerns the gene B3GNT3 and esophageal squamous cell carcinoma.