The expression of B3GNT3 in ESCA cells was detected by QRT-PCR, and compared with normal cells, it was found that the expression of B3GNT3 in two ESCC cell lines were higher than that in normal HEEC cell line (p < 0.05, Fig. 1a). This evidence concerns the gene B3GNT3 and esophageal squamous cell carcinoma.