Cytotoxicity of the current l-asparaginase against various human cancer cell lines was investigated, and the results revealed that the enzyme was toxic to HepG-2 (67%) with IC50 of 21.2 μg/mL, and MCF-7 (38%) with IC50 of 34.2 μg/mL, while it was not effective against Hct-116 and DMEM A-549. Here, ASPG is linked to cancer.