2017; Prasad et al. 2020). Therefore, the drug targeting cancer cell stemness is beneficial for cancer treatment. In this research, we studied the action of vitexin on the stemness capacity of endometrial cancer. It was revealed that vitexin retrained the sphere formation ability. Besides, the expression levels of OCT4 and Nanog were also inhibited by vitexin. OCT4 and Nanog are the stemness biomarkers, and they play crucial roles in maintaining stem cell pluripotency and proliferation ability (Zhang et al. 2013; Bai et al. 2015). The gene discussed is POU5F1; the disease is endometrial cancer.