To better study the characteristics of the SULT1E1+ subpopulation, we developed a novel protocol for establishing a patient‐derived MO model based on that outlined by Jacob et al.[31] Compared to the protocols for other MO models reported previously,[32] one major advantage of our protocol is that it avoids the enzymatic digestion of tumor samples into single cells, thereby ensuring the preservation of most cell types in the MO model. The gene discussed is SULT1E1; the disease is neoplasm.