Briefly, after anesthesia with isoflurane, the mice were injected in the left hepatic lobe with PBS (referred to as the sham-operation group), 5 × 106 wildtype (WT) Hepa1-6 tumor cells (referred to as the control group), or 5 × 106Fgl1-KO Hepa1-6 tumor cells (referred to as the sh-Fgl1 group). Here, FGL1 is linked to neoplasm.