Albeit no difference in the density of immune cell subsets positive for markers in the pretreatment specimens between responders and non-responders, responders displayed significantly increased infiltration of CD8+ T cells in the tumor, CD20+ T cells, PD-1+ cells, PD-L1+ cells, and PD-L1+CD68+ macrophages in the stroma; CD4+Foxp3+ Treg cell and PD-L1+CD68+ macrophage density in the stroma significantly increased in non-responders. The gene discussed is CD4; the disease is neoplasm.