NACT significantly increased infiltrates of CD4+Foxp3+ Treg cells (P = 0.036) and PD-L1+CD68+ macrophages (P = 0.022) in the stroma and decreased M1/M2 ratio (P = 0.016) in the tumor of CRS1 non-responders (Figures 2H, J, K). This evidence concerns the gene CD4 and neoplasm.