CYP2E1 and cancer: To test our hypothesis, ferroptosis was induced with class 2 inducers (RSL-3 or ML-162) in mammalian COS-7 cancer cells that don’t express CYP2E1, and in cells engineered to express human CYP2E1, and the impact on viability, lipid peroxidation, GPX4, and GSH levels was assessed.