To determine if Merlin regulates Wnt signaling in meningioma cells, M10G human meningioma cells stably expressing CRISPR interference (CRISPRi) machinery (dCas9-KRAB)26,27 were transduced with non-targeted control sgRNAs (sgNTC) or sgRNAs suppressing endogenous NF2(sgNF2) (Extended Data Fig. 4a). This evidence concerns the gene NF2 and meningioma.