To validate these findings, IOMM-Lee human meningioma cells encoding endogenous Merlin22 were transduced with short-hairpin RNAs (shRNAs) suppressing NF2 (shNF2), or non-targeted control shRNAs (shNTC), and grown as xenografts in mice (Extended Data Fig. 2a). This evidence concerns the gene NF2 and meningioma.