NF2 and meningioma: Subcellular fractionation of CH-157MN cells after doxycycline-inducible Merlin rescue showed β-catenin was distributed across the plasma membrane, cytoplasm, cytoskeleton, and nucleus with MerlinL46R and MerlinA211D rescue, but was only enriched at the plasma membrane and in the nucleus with MerlinWT rescue (Fig. 2d), β-catenin suppression using siRNAs (siCTNNB1) inhibited meningioma Wnt signaling (Fig. 2e and Extended Data Fig. 5c), but Merlin was required for maximal Wnt pathway activation in meningioma cells even after β-catenin overexpression (Fig. 2f and Extended Data Fig. 5d).