After subsetting 7 days KPY tumor organoids into CD44-High (enriched for Hmga2-high cells) and CD44-Neg (enriched for SPC-high cells) populations, we cultured those cells into organoids with (co-culture) or without (mono-culture) lung mesenchymal cells to compare the organoid forming efficiency (OFE) (Figure 4D) ; cells from established organoids were separated based on CD44 staining and replated into cultures either with or without mesenchymal cells. This evidence concerns the gene HMGA2 and neoplasm.