Probiotics have emerged as a safe and effective option for treating HFD-induced obesity in animals, with few adverse effects and good tolerance, making them ideal for long-term administration (Liu et al., 2017), and the combination of Lactobacillus and Bifidobacterium has been shown to significantly alter gut microbiota composition and improve insulin sensitivity in HFD mice. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.