However, although both kinases were efficiently cleaved in vitro, only endogenous RIPK1 was cleaved in a Jurkat cell line upon doxycycline-mediated induction of HIV-1 PR and in HEK293T cells infected with a VSVg pseudotyped HIV-1 NL4.3 and Sup-T1 cells infected with replication-competent HIV-1 NL4.3 at 24 and 48 h post-infection, respectively. The gene discussed is RIPK1; the disease is infection.