S100A8 and gastric cancer: Supplementary Figure S5G shows the decreased concentration of IL-8 obtained in the conditioned media of the AGS cells transfected with the specific IL-8-siRNA. The reduction was of approximately 70% in cells infected with the CagA(+) ABD strain. We observed a reduction of approximately 65% in the capacity of the IL-8-silenced AGS conditioned media to chemoattract Akata cells (Figure 4F). We further explored whether IL-8 expression levels in the TCGA transcriptomic database would influence the survival of patients with GC, without finding an association (Supplementary Figure S5H).