Additionally, Reactome analysis suggested that the target genes of multiple miRNAs in EVs were associated with cellular senescence, MAPK family signaling cascades, constitutive signaling by EGFRvIII, signaling by EGFRvIII in cancer, GRB2 events in ERBB2 signaling, regulation of RUNX1 expression and activity, cellular responses to stress, oncogene-induced senescence, mitotic G1-G1/S phases, MAPK6/MAPK4 signaling, as well as VEGFA-VEGFR2 pathway, signaling by VEGF, and interleukin-4 and 13 signaling. The gene discussed is MAPK4; the disease is cancer.