In the current study, we further characterized the binding capacity of annexin A5 and GlaS to different EV classes, small EVs (sEVs; <200 nm in diameter) and medium/large EVs (m/lEVs; >200 nm in diameter), categorized according to the Minimal Information for Studies of Extracellular Vesicles (MISEV) 2018 guidelines [24], derived from cancer and non-cancerous cell types. The gene discussed is ANXA5; the disease is cancer.