At 36 dpi, 27 DEmRNAs (e.g. hap1, clta and dctn4) were significantly enriched in 12 pathways, including pentose and glucuronate interconversions, starch and sucrose metabolism, ascorbate and aldarate metabolism, endocrine and other factor-regulated calcium reabsorption, alcoholism, sulfur relay system, drug metabolism-other enzymes, chemical carcinogenesis, Huntington's disease, porphyrin and chlorophyll metabolism, long-term depression and circadian entrainment (Fig. 4c and Additional file 6: Table S4). Here, DCTN4 is linked to juvenile Huntington disease.