PTEN and pachyonychia congenita: To further investigate the molecular impact of suppressing MBTPS2 expression in PC we carried out transcriptomic analysis, selecting LNCaP cells as the cell model of choice based on their lack of PTEN expression (as in our SB:PtenNull model) and the observed phenotypic changes on knockdown of MBTPS2. Principle component analysis (PCA) demonstrated that LNCaP cells cluster separately following knockdown of MBTPS2 compared to control cells (Supplementary Fig. 5A, B), with minimal variance between experimental replicates within each condition.