Enhanced proteome-levels of AMPK signaling (e.g., PRKAA1 and PPP2R5E) and insulin resistance (e.g., CAB39L and RAB13) were detected in the D2 (LGIN stage), and elevated cell cycle (e.g., CDK1 and RB1) and DNA repair (e.g., RFC1 and MSH3) in the D3 (HGIN stage), suggesting a response to exogenous stresses. The gene discussed is PRKAA1; the disease is Insulin resistance.