To confirm the tumor-promoting function of PFKFB3 in TC, we used two different siRNAs to knock down PFKFB3 in KTC-1 and found that PFKFB3 knockdown significantly increased the ADP/ATP ratio and reduced the phosphorylation level of ERK and AKT (Supplementary Fig. 4A, B). Here, AKT1 is linked to neoplasm.