We did not observe discordant variant calls for any of the genes associated with atrial fibrillation including SNC5A, KCNQ1, KCNH2, TBX5, GJA5, MYL4, TTN, and KCNA5, whereas we found a few discordant variant calls in some of the cardiac genes on the ACMG list, including DSG2, DSP, LMNA, MYH7, and TPM1. The gene discussed is TPM1; the disease is atrial fibrillation.