Western blot analysis of bile samples from 24 cats revealed that 10 samples from cats with AA-amyloidosis and 5 samples from cats without AA-amyloidosis were SAA-positive, respectively (Fig 3, S4 Fig; negative control for secondary antibody in S5 Fig) showing a band with a molecular mass of approximately 8 kDa. The gene discussed is SAA1; the disease is AA amyloidosis.