The results revealed that except for S100A11 (Figure 6C), the expression of COL1A2, MMP9, VIM and S100A4 significantly affected the overall survival time of patients with ES, in which the OS time of the high expression group of MMP9 and COL1A2 was significantly lower than that of the low expression group (Figure 6D, 6E), and the OS time of the low expression group of VIM and S100A4 was significantly lower than that of the high expression group (Figure 6F, 6G). This evidence concerns the gene S100A4 and Ewing sarcoma.