To figure out the functional roles of LncFEZF1-AS1 in RCC, the FEZF1-AS1 knockdown vector was first successfully constructed, and then transfected into ACHN and 769-P cells to obtain RCC cell lines with stable FEZF1-AS1 knockdown expression (Figure 2A). The gene discussed is FEZF1; the disease is renal cell carcinoma.