CCK-8 and EdU experiments both showed that, the RCC cell proliferation was increased after co-transfection of FEZF1-AS1 and ETNK1 knockdown vectors, compared with the single transfection of FEZF1-AS1 knockdown vector (Figure 4B, C), suggesting that knockdown of ETNK1 can reverse the effect of LncFEZF1-AS1 on the proliferative ability of RCC cells. The gene discussed is ETNK1; the disease is renal cell carcinoma.