Irradiated (with a laser at 670 nm at 0.25 W/cm2 for 10 min) PCN-ACF-CpG@HA (10 mg/kg) treatment of H22-bearing mice demonstrated inhibition of hypoxia-induced cell survival and metastasis signaling genes, persistent high DC maturation (61.21%), and subsequent increase in CD8+ T cell and CD4+ T cell infiltration at the tumor site, resulting in efficient tumor suppression and metastasis prevention. Here, CD4 is linked to neoplasm.