PTPN1 and diabetes mellitus: Acankoreagenin (16a) demonstrated inhibitory activities towards different enzymes responsible for diabetes, such as α-glucosidase and protein tyrosine phosphatase 1B (PTP1B), displaying IC50 ∼ 13 and 16 μM, respectively, and towards α-amylase (in vitro; IC50 = 31 μM).