PLIN1 and neuroblastoma: To test whether B. breve MCC1274-derived metabolites affected perilipin gene expression, SH-SY5Y human neuroblastoma cells were exposed to the two cell extracts (HKs and Sonic) at final concentration of 1% v/v for 1 h, total RNA was extracted, and RT-PCR was conducted to analyze the changes in perilipin mRNA expression.