In this sense, TLR9 is significantly more expressed in Leishmania (L.) amazonensis infections in severe anergic diffuse cutaneous leishmaniasis (ADCL) in comparison to localized cutaneous leishmaniasis (LCL) caused by the same leishmanine species [50], and TLR2 mediates non-protective immune response in L. (L.) amazonensis and Leishmania (V.) braziliensis infected mice, in contrast to L. (L.) infantum-infection [45]. This evidence concerns the gene TLR9 and diffuse cutaneous Leishmaniasis.