Next, we examined the expression of several Mreg markers, such as CD163, CD206, CD209, CD274, and MER-TK [31], and tumor-associated macrophage (TAM) marker TREM2, which exerts anti-inflammatory functions [32] by flow cytometry following culture of PMA-treated-THP-1 cells on different types of PDA- or PDA+RGD-coated 3D micropatterns. Here, CD274 is linked to neoplasm.