Acute inflammation in both cases did not influence ROS base level, indicating that HDF promoted the amplification of ROS production by granulocytes even in the absence of obesity signs in mice, possibly supporting NADPH-oxidase in an active state or stimulating lipid and lipoprotein peroxidation [44,45,46]. This evidence concerns the gene FMO5 and obesity due to melanocortin 4 receptor deficiency.