High-throughput sequencing and bioinformatics analysis showed that MEF2B, CCDC187, PSD2, RGS16, PLXDC1, ARV1, HELB, ASIC3, PLCH2, IGF2BP3, CLHC1, DNHD1, SACS, H1-4, ANKRD36, and ZNF117 could be the targets of action of rotundine to inhibit colorectal cancer, which may be for further provide new biomarkers for the diagnosis and treatment of colorectal cancer. This evidence concerns the gene HELB and colorectal cancer.