This was conducted using a BD FACSAria II Cell Sorter and BD FACSMelody, which can sort populations of CD44/CD117/CD133 triple-marker positive and ALDH-positive populations from head and neck cancer cells from four cultured primary cells and four immortalized cell lines (SSC28, FaDu, HN13, and HEP-2). This evidence concerns the gene CD44 and head and neck cancer.