Our results showed the presence of typical small EVs proteins, such as Alix (Invitrogen), TSG101 (Invitrogen), and CD63 (Invitrogen), while α-Tubulin (Invitrogen) and HSP-90 (CUSABIO) were only found in cell lysates (Figure 1B), in contrast to other results obtained with small EVs purified from breast or prostate cancer cells [29,30]. Here, TSG101 is linked to Familial prostate cancer.