In addition, by expression analysis of the Hh signaling- and autophagy-related genes in brain tumors of a GBM zebrafish model and the evaluation of the Lc3b signal in the model with Hh/Hdac6 dysregulation, we provided data supporting the effects of the combination of Hh and HDAC6 inhibition in cancer treatment. The gene discussed is HDAC6; the disease is cancer.