Furthermore, our study observed several differentially regulated EFP proteins in the AE group that can mitigate diet-induced obesity, such as the upregulated PRDX5 [34] as well as the downregulated LAMA4 [35], S27A1 [36], and HS12A [37], as reported in previous studies. Here, TRIM25 is linked to obesity due to melanocortin 4 receptor deficiency.