The confocal images showed that the αSMA signal was distributed at the cytoplasmic level throughout the fibroblasts, and its signal was significantly higher in the 3D CRC μTs (Figure 7B,C, white arrows) than in the 3D Stroma μTs, where the signal was almost absent (Figure 7E,F). The gene discussed is ACTA1; the disease is colorectal carcinoma.