It has been reported that treatment of HCT116 and HT29 colon cancer cells with a natural naphthoquinone 2-methoxy-6-acetyl-7-methyljuglone (MAM), isolated from Polygonum cuspidatu, validated the role of JNK in MAM-induced necroptosis, marked by mitochondrial depolarization, ATP depletion, and an increased production of mitochondrial ROS [48]. This evidence concerns the gene MAPK8 and malignant colon neoplasm.