The results demonstrated that the depleted DUSP6 compensated for the decrease in Chk2 and phospho-Chk2 caused by the high expression of RIG-I, and the expression of the G2/M checkpoint protein cyclinB1 and phospho-cdc2 was lowered after irradiation (Figure 7E), which restored the radiosensitivity of ESCC cells. This evidence concerns the gene RIGI and esophageal squamous cell carcinoma.