To circumvent these problems, in our study we used antibodies to detect several key components of the hyaluronan-binding neural ECM, namely the lecticans brevican, neurocan, aggrecan and the link protein HAPLN1/Crtl1, in subcellular fractions from three different post-mortem brain areas, i.e., hippocampus, temporal and frontal cortex, to provide a comprehensive and more differentiated picture of subcellular ECM rearrangement during AD in the human brain. This evidence concerns the gene HAPLN1 and Alzheimer disease.