The CD9 is a classical tetraspanin, used in several studies to characterize EVs including in the context of exosomes of dogs serum characterization [26], CD5L is a marker previously shown to be present in human plasma-derived EVs and in the context of isolation of EVs from a patient with Chagas disease [17,27] and CD71 is an exosomal marker previously used in the context of characterization of plasma-derived EVs in Plasmodium vivax infected patients [28,29]. Here, CD9 is linked to Chagas disease.