Here, we found that (i) the anti-EGFR single-chain antibody, PD1 single-chain antibody (E27), and CCR6 were stably expressed on the surface of EGFR-CAR T cells; (ii) these EGFR CAR T cells possessed a higher proportion of central memory T cells and initial T cells with higher cytotoxicity; and (iii) they possessed in vitro- and in vivo-specific and potent cytotoxicity against NSCLC cells, especially EGFR CAR-E27-CCR6-T. The gene discussed is CCR6; the disease is non-small cell lung carcinoma.