CALCA and migraine disorder: CGRP, substance P (SP), PACAP, vasoactive intestinal peptide (VIP), and neuropeptide Y (NPY) are implicated in craniocervical vasodilatation, with SP also having a role in plasma protein extravasation and CGRP and PACAP in peripheral and/or central sensitization, the main mechanisms related to migraine pathophysiology [23], whereas glutamate has been linked to neuronal hyperexcitability and plays a pivotal role in triggering migraine attacks [24].