TNF and primary biliary cholangitis: Flow cytometry analysis showed that in the serum of PBC patients at an age of diagnosis over 65, the level of IL-8 was significantly lower (40% reduction, p = 0.04; Figure 2A) while the level of TNF-α was significantly higher (20% elevation, p = 0.006; Figure 2B) in comparison to these levels in PBC patients at an age of diagnosis under 40.