In a tumor model based on harvested macrophages and breast cancer cells from Swiss albino mice, hBD-2 treatment of the macrophages increased the expression of inflammatory cytokines (IFN-γ, interleukin 1α (IL-1α) and tumor necrosis factor α (TNF-α)) and chemokines (CXCL1, CXCL5 and CCL5). This evidence concerns the gene TNF and breast cancer.