Further analysis of the expression of the amyloid precursor protein (APP) and Aβ1–42 in brain homogenates by western blot revealed that APP expression levels were significantly reduced in treated 3xTg AD mice in comparison with untreated 3xTg AD animals and, once again, this effect was more evident in the group treated with a lower extract dose (Figure 2B–D). This evidence concerns the gene APP and Alzheimer disease.