To investigate the effect on tumorigenesis after FXR KO in vivo, we first determined FXR protein expression in different hepatocellular carcinoma cell lines (Figure 1D) and selected Hep3B cells to construct an FXR knockout hepatocellular carcinoma cell line (FXR KO Hep3B) using the CRISPR/Cas9 system. Here, NR1H4 is linked to hepatocellular carcinoma.